Hotspot

rbcL-rbcS

LSC · 51295-51574 · 280 bp

Back to Ecklonia

Pi 0.0238

MarkerSeek score 53.20

Primer available yes

Label rbcL-rbcS

Feature Metadata

label_name: rbcL-rbcS
feature_type: igs
region: LSC
length_bp: 280
variable_sites: 10
pi: 0.0238
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-rbcS_p1	TCCTGTAGCTTCTGGTGGAA	ACCAGGTTCGTTTGAAGGTC	50.0	50.0	58.0	58.0	935-938 bp	1.0	81.3
rbcL-rbcS_p2	TTCCTGTAGCTTCTGGTGGA	ACCAGGTTCGTTTGAAGGTC	50.0	50.0	58.0	58.0	936-939 bp	1.0	81.3
rbcL-rbcS_p3	CTATCTGGGCACGAAAAGCT	ACCAGGTTCGTTTGAAGGTC	50.0	50.0	58.0	58.0	1218-1221 bp	1.0	80.9
rbcL-rbcS_p4	GTTCCTGTAGCTTCTGGTGG	ACCAGGTTCGTTTGAAGGTC	55.0	50.0	57.9	58.0	937-940 bp	1.0	80.7
rbcL-rbcS_p5	TGGTCCTTTAAAAGCAGCGT	ACCAGGTTCGTTTGAAGGTC	45.0	50.0	58.0	58.0	707 bp	0.666667	67.8