Hotspot

rpl20-ilvH

LSC · 47822-47908 · 87 bp

Back to Ecklonia

Pi 0.023

MarkerSeek score 65.10

Primer available yes

Label rpl20-ilvH

Feature Metadata

label_name: rpl20-ilvH
feature_type: igs
region: LSC
length_bp: 87
variable_sites: 3
pi: 0.023
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-ilvH_p1	ACCCTTCAGCTTTTTCTGTCA	AATCCTCCGGCATCCTTTTC	42.9	50.0	57.7	57.9	171-175 bp	1.0	72.4
rpl20-ilvH_p2	ACCCTTCAGCTTTTTCTGTCA	CTCCGGCATCCTTTTCAACT	42.9	50.0	57.7	58.2	167-171 bp	1.0	71.5
rpl20-ilvH_p3	ACCCTTCAGCTTTTTCTGTCA	GAACTAATCCTCCGGCATCC	42.9	55.0	57.7	57.8	176-180 bp	1.0	71.4
rpl20-ilvH_p4	ACCCTTCAGCTTTTTCTGTCA	ACTAATCCTCCGGCATCCTT	42.9	50.0	57.7	58.2	174-178 bp	1.0	71.3
rpl20-ilvH_p5	ACCCTTCAGCTTTTTCTGTCA	AACTAATCCTCCGGCATCCT	42.9	50.0	57.7	58.2	175-179 bp	1.0	71.2