Hotspot

trnM-trnD

LSC · 38635-38791 · 157 bp

Back to Ecklonia

Pi 0.017

MarkerSeek score 56.50

Primer available yes

Label trnM-trnD

Feature Metadata

label_name: trnM-trnD
feature_type: igs
region: LSC
length_bp: 157
variable_sites: 4
pi: 0.017
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-trnD_p1	CGACCAAGGGCTTATGAGTC	GAATTTCGGGATTGACGGGA	55.0	50.0	58.1	58.0	287 bp	1.0	80.4
trnM-trnD_p2	GGGCTTATGAGTCCCCTACT	GAATTTCGGGATTGACGGGA	55.0	50.0	57.9	58.0	280 bp	1.0	80.0
trnM-trnD_p3	TCTGGCTGGATTTGAACCTG	GAATTTCGGGATTGACGGGA	50.0	50.0	57.8	58.0	307 bp	1.0	79.1
trnM-trnD_p4	ACTCTGGCTGGATTTGAACC	GAATTTCGGGATTGACGGGA	50.0	50.0	57.8	58.0	309 bp	1.0	79.1
trnM-trnD_p5	ACTCAGCCATCTCACCCTAA	GAATTTCGGGATTGACGGGA	50.0	50.0	57.7	58.0	334 bp	1.0	78.7