Hotspot

dnaK-trnS

LSC · 38331-38464 · 134 bp

Back to Ecklonia

Pi 0.0498

MarkerSeek score 72.50

Primer available yes

Label dnaK-trnS

Feature Metadata

label_name: dnaK-trnS
feature_type: igs
region: LSC
length_bp: 134
variable_sites: 10
pi: 0.0498
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaK-trnS_p1	CTCCAAATATTTTACTTATATTAGCCA	TGAGATGGCTGAGTGGCTTA	25.9	50.0	54.0	58.4	249-250 bp	1.0	43.5
dnaK-trnS_p2	CTCCAAATATTTTACTTATATTAGCCA	TTAGGGTGAGATGGCTGAGT	25.9	50.0	54.0	57.7	255-256 bp	1.0	43.4
dnaK-trnS_p3	ACTCCAAATATTTTACTTATATTAGCC	TTAGGGTGAGATGGCTGAGT	25.9	50.0	54.0	57.7	256-257 bp	1.0	43.4
dnaK-trnS_p4	CTCCAAATATTTTACTTATATTAGCCA	GGTTCAAATCCAGCCAGAGT	25.9	50.0	54.0	57.8	280-281 bp	1.0	43.1
dnaK-trnS_p5	ACTCCAAATATTTTACTTATATTAGCC	GGTTCAAATCCAGCCAGAGT	25.9	50.0	54.0	57.8	281-282 bp	1.0	43.1