Hotspot

thiG-trnG

LSC · 35869-35997 · 129 bp

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Pi 0.0362

MarkerSeek score 67.50

Primer available yes

Label thiG-trnG

Feature Metadata

label_name: thiG-trnG
feature_type: igs
region: LSC
length_bp: 129
variable_sites: 7
pi: 0.0362
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
thiG-trnG_p1	CAGTCCCAACAATAAGGCGA	AACTTGCGAGTGTAGCTCAG	50.0	50.0	57.9	57.9	273 bp	1.0	80.6
thiG-trnG_p2	GTCCCAACAATAAGGCGAGA	AACTTGCGAGTGTAGCTCAG	50.0	50.0	57.6	57.9	271 bp	1.0	78.3
thiG-trnG_p3	AGTCCCAACAATAAGGCGAG	AACTTGCGAGTGTAGCTCAG	50.0	50.0	57.6	57.9	272 bp	1.0	78.3
thiG-trnG_p4	TCCAGTCCCAACAATAAGGC	AACTTGCGAGTGTAGCTCAG	50.0	50.0	57.5	57.9	275 bp	1.0	77.4
thiG-trnG_p5	CAGTCCCAACAATAAGGCGA	CTTGCGAGTGTAGCTCAGTG	50.0	55.0	57.9	58.7	271 bp	1.0	76.0