Hotspot

rpoC2-rpoC1

LSC · 18863-18875 · 13 bp

Back to Ecklonia

Pi 0.0513

MarkerSeek score 70.60

Primer available yes

Label rpoC2-rpoC1

Feature Metadata

label_name: rpoC2-rpoC1
feature_type: igs
region: LSC
length_bp: 13
variable_sites: 1
pi: 0.0513
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rpoC1_p1	GGCAGCTTTTCTCTGACCAT	TCGAACTACACCTGGTCGTA	50.0	50.0	58.2	57.8	179 bp	1.0	77.6
rpoC2-rpoC1_p2	GGCAGCTTTTCTCTGACCAT	ATTCGAACTACACCTGGTCG	50.0	50.0	58.2	57.4	181 bp	1.0	73.9
rpoC2-rpoC1_p3	GGCAGCTTTTCTCTGACCAT	TTCGAACTACACCTGGTCGT	50.0	50.0	58.2	58.7	180 bp	1.0	73.7
rpoC2-rpoC1_p4	TAGGCAGCTTTTCTCTGACC	TCGAACTACACCTGGTCGTA	50.0	50.0	57.2	57.8	181 bp	1.0	72.8
rpoC2-rpoC1_p5	GGCAGCTTTTCTCTGACCATA	TCGAACTACACCTGGTCGTA	47.6	50.0	58.0	57.8	179 bp	1.0	70.9