Hotspot

trnN-GUU-trnR-ACG

IRa · 125488-126081 · 594 bp

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Pi 0.0135
MarkerSeek score 66.40
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
594
variable_sites
8
pi
0.0135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 735 bp 1.0 79.1
trnN-GUU-trnR-ACG_p2 CCGTTGTTCCTCAGTAGCTC GAACAGATCCAGTGGAGACG 55.0 55.0 58.0 57.7 772 bp 1.0 78.5
trnN-GUU-trnR-ACG_p3 TGGGTTGAGAACGGGAATTG CAGAGGATTAGAGCACGTGG 50.0 55.0 58.1 57.8 776 bp 1.0 78.3
trnN-GUU-trnR-ACG_p4 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 50.0 55.0 58.2 57.8 686 bp 1.0 78.0
trnN-GUU-trnR-ACG_p5 TGGGTTGAGAACGGGAATTG GAACAGATCCAGTGGAGACG 50.0 55.0 58.1 57.7 813 bp 1.0 77.7