Hotspot

rpl32-trnL-UAG

SSC · 109749-110283 · 535 bp

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Pi 0.043
MarkerSeek score 68.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
535
variable_sites
23
pi
0.043
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAAGGGGTATTGGACAGCG GCTGAAGAAAAACGAGGCAC 50.0 50.0 58.2 58.0 1373 bp 0.5 58.4
rpl32-trnL-UAG_p2 AAAAGGGGTATTGGACAGCG CTTAGTGGAAAATGCCCCGA 50.0 50.0 58.2 57.9 1021 bp 0.5 57.7
rpl32-trnL-UAG_p3 AAAAGGGGTATTGGACAGCG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 672 bp 0.5 57.7
rpl32-trnL-UAG_p4 AAAAGGGGTATTGGACAGCG ACTTAGTGGAAAATGCCCCG 50.0 50.0 58.2 58.2 1022 bp 0.5 57.3
rpl32-trnL-UAG_p5 AAAAGGGGTATTGGACAGCG AGCAGCGTGTCTACCAATTT 50.0 45.0 58.2 57.8 665 bp 0.5 57.2