Hotspot

petG-trnW-CCA

LSC · 63927-64052 · 126 bp

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Pi 0.0763
MarkerSeek score 88.40
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
126
variable_sites
9
pi
0.0763
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
88.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 TTTACAATACAGGCGGGGTG AGAACGTGGGTCTCCAAAAC 50.0 50.0 57.9 58.0 208-216 bp 1.0 85.2
petG-trnW-CCA_p2 TTTACAATACAGGCGGGGTG CAAAACCCGATGTCGTAGGT 50.0 50.0 57.9 57.9 194-202 bp 1.0 85.2
petG-trnW-CCA_p3 TTTACAATACAGGCGGGGTG AGCGCTCTTAGTTCAGTTCG 50.0 50.0 57.9 58.0 230-238 bp 1.0 84.7
petG-trnW-CCA_p4 TTTACAATACAGGCGGGGTG CCCGATGTCGTAGGTTCAAA 50.0 50.0 57.9 57.6 189-197 bp 1.0 83.1
petG-trnW-CCA_p5 TTTACAATACAGGCGGGGTG CGCTCTTAGTTCAGTTCGGT 50.0 50.0 57.9 57.7 228-236 bp 1.0 82.1