Hotspot

petL-petG

LSC · 63641-63812 · 172 bp

Back to Echium

Pi 0.0174
MarkerSeek score 55.70
Primer available yes
Label petL-petG

Feature Metadata

label_name
petL-petG
feature_type
igs
region
LSC
length_bp
172
variable_sites
3
pi
0.0174
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petL-petG_p1 TTCGGTTTTCTACTAGCGGC CACCCCGCCTGTATTGTAAA 50.0 50.0 58.0 57.9 344 bp 1.0 79.8
petL-petG_p2 CGGTTTTCTACTAGCGGCTT CACCCCGCCTGTATTGTAAA 50.0 50.0 58.0 57.9 342 bp 1.0 79.7
petL-petG_p3 TTCGGTTTTCTACTAGCGGC ATCACCCCGCCTGTATTGTA 50.0 50.0 58.0 58.5 346 bp 1.0 76.7
petL-petG_p4 CGGTTTTCTACTAGCGGCTT ATCACCCCGCCTGTATTGTA 50.0 50.0 58.0 58.5 344 bp 1.0 76.7
petL-petG_p5 TTCGGTTTTCTACTAGCGGC AAAGGTCCAACTGATCACCC 50.0 50.0 58.0 57.4 359 bp 1.0 76.0