Hotspot

petA-psbJ

LSC · 60892-61672 · 781 bp

Back to Echium

Pi 0.0196
MarkerSeek score 72.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
781
variable_sites
15
pi
0.0196
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CCCATTACGTGTTCAAAGCC ATTCCGGATTGGGTTCATCC 50.0 50.0 57.4 57.6 909-925 bp 1.0 74.7
petA-psbJ_p2 GCCTTTTGTTCTTCTTGGCA ATTCCGGATTGGGTTCATCC 45.0 50.0 57.1 57.6 892-908 bp 1.0 72.7
petA-psbJ_p3 AGCCTTTTGTTCTTCTTGGC ATTCCGGATTGGGTTCATCC 45.0 50.0 56.8 57.6 893-909 bp 1.0 70.3
petA-psbJ_p4 GACCCATTACGTGTTCAAAGC ATTCCGGATTGGGTTCATCC 47.6 50.0 57.8 57.6 911-927 bp 1.0 70.2
petA-psbJ_p5 ACGTGTTCAAAGCCTTTTGT ATTCCGGATTGGGTTCATCC 40.0 50.0 56.7 57.6 903-919 bp 1.0 69.3