Hotspot

trnT-UGU-trnL-UAA

LSC · 45568-46179 · 612 bp

Back to Echium

Pi 0.0209
MarkerSeek score 61.30
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
612
variable_sites
12
pi
0.0209
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 TAATCCTGGTAAAGCCCCCA GACCAAAGCAGTCAACTCCA 50.0 50.0 58.0 58.0 1324-1375 bp 1.0 83.6
trnT-UGU-trnL-UAA_p2 AAAATGGGAAAAGCCGGCTA GACCAAAGCAGTCAACTCCA 45.0 50.0 58.1 58.0 926-970 bp 1.0 83.6
trnT-UGU-trnL-UAA_p3 TCTAAGATCACTCCCGGGTC GACCAAAGCAGTCAACTCCA 55.0 50.0 57.9 58.0 1360-1411 bp 1.0 83.0
trnT-UGU-trnL-UAA_p4 AAGATCACTCCCGGGTCTAG GACCAAAGCAGTCAACTCCA 55.0 50.0 57.9 58.0 1357-1408 bp 1.0 83.0
trnT-UGU-trnL-UAA_p5 CGAAGCCATACCCAATCGAA GACCAAAGCAGTCAACTCCA 50.0 50.0 58.1 58.0 1567-1618 bp 1.0 82.7