Hotspot

atpA-atpF

LSC · 10812-10867 · 56 bp

Pi 0.06

MarkerSeek score 71.40

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 56
variable_sites: 3
pi: 0.06
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ATGCCATCGCCTACTTGAAG	GTGGAAATAGAAGCCGAGCA	50.0	50.0	58.0	58.0	460-466 bp	1.0	81.6
atpA-atpF_p2	AAGTGGTTACTACCTGGGCT	GTGGAAATAGAAGCCGAGCA	50.0	50.0	58.0	58.0	981-987 bp	1.0	80.8
atpA-atpF_p3	TCGGCTCGAATGGTTACCA	GCGATGAAAGAAATAATTGATTAG	52.6	29.2	58.7	53.0	94-100 bp	1.0	46.5
atpA-atpF_p4	CGGCTCGAATGGTTACCA	GCGATGAAAGAAATAATTGATTAG	55.6	29.2	57.1	53.0	93-99 bp	1.0	46.5
atpA-atpF_p5	TCGGCTCGAATGGTTACC	GCGATGAAAGAAATAATTGATTAG	55.6	29.2	56.8	53.0	94-100 bp	1.0	46.5