Hotspot

trnS-GCU-trnG-UCC

LSC · 7490-8218 · 729 bp

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Pi 0.0434
MarkerSeek score 76.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
729
variable_sites
30
pi
0.0434
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA GGGGTAATAGAACGAATCACACT 45.0 43.5 58.0 58.0 798-814 bp 1.0 62.2
trnS-GCU-trnG-UCC_p2 CCATCAACGGAAAGAGAGGG GGGGTAATAGAACGAATCACACT 55.0 43.5 58.0 58.0 853-869 bp 1.0 61.8
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA GGGGTAATAGAACGAATCACACT 50.0 43.5 58.2 58.0 847-863 bp 1.0 60.9
trnS-GCU-trnG-UCC_p4 AGCCATCAACGGAAAGAGAG GGGGTAATAGAACGAATCACACT 50.0 43.5 57.6 58.0 855-871 bp 1.0 58.9
trnS-GCU-trnG-UCC_p5 CCATCAACGGAAAGAGAGGG GGGGTAATAGAACGAATCACAC 55.0 45.5 58.0 56.6 853-869 bp 1.0 58.8