Hotspot

trnK-UUU-rps16

LSC · 4113-4812 · 700 bp

Back to Echium

Pi 0.0215
MarkerSeek score 59.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
700
variable_sites
15
pi
0.0215
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC GCTTCTCCCTAATCAAACGGA 55.0 47.6 57.9 57.7 824-826 bp 1.0 71.0
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC AGCTTCTCCCTAATCAAACGG 55.0 47.6 57.9 57.7 825-827 bp 1.0 71.0
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GCTTCTCCCTAATCAAACGGA 50.0 47.6 58.2 57.7 763-765 bp 1.0 70.4
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GCTTCTCCCTAATCAAACGGA 50.0 47.6 58.2 57.7 762-764 bp 1.0 70.4
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AGCTTCTCCCTAATCAAACGG 50.0 47.6 58.2 57.7 763-765 bp 1.0 70.4