Hotspot

ycf58

Genome · 67634-68047 · 414 bp

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Pi 0.14

MarkerSeek score 75.20

Primer available yes

Label ycf58

Feature Metadata

label_name: ycf58
feature_type: gene
region: Genome
length_bp: 414
variable_sites: 58
pi: 0.14
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf58_p1	ATCAGCAAAAGCCCTTTCCT	AGCACGCGGTTATAAGACAG	45.0	50.0	57.7	58.1	1317 bp	0.5	56.9
ycf58_p2	ATCAGCAAAAGCCCTTTCCT	GAGATGTTAGCACGCGGTTA	45.0	50.0	57.7	58.1	1325 bp	0.5	56.8
ycf58_p3	ATCAGCAAAAGCCCTTTCCT	GCACGCGGTTATAAGACAGA	45.0	50.0	57.7	58.1	1316 bp	0.5	56.8
ycf58_p4	CAGCAAAAGCCCTTTCCTCT	AGCACGCGGTTATAAGACAG	50.0	50.0	58.4	58.1	1315 bp	0.5	56.4
ycf58_p5	TCAGCAAAAGCCCTTTCCTC	AGCACGCGGTTATAAGACAG	50.0	50.0	58.4	58.1	1316 bp	0.5	56.3