Hotspot

ycf53

Genome · 14265-14987 · 723 bp

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Pi 0.0941

MarkerSeek score 78.50

Primer available yes

Label ycf53

Feature Metadata

label_name: ycf53
feature_type: gene
region: Genome
length_bp: 723
variable_sites: 68
pi: 0.0941
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf53_p1	TCATCCAGAGGCTAGTCCAG	GCGAACGACAAAAAGTATGGA	55.0	42.9	57.9	57.2	1175 bp	0.5	45.0
ycf53_p2	TCATCCAGAGGCTAGTCCAG	AGCGAACGACAAAAAGTATGG	55.0	42.9	57.9	57.2	1176 bp	0.5	44.9
ycf53_p3	TCAAAATCACGGTTTTGCGG	GCGAACGACAAAAAGTATGGA	45.0	42.9	57.9	57.2	1319 bp	0.5	44.6
ycf53_p4	TCAAAATCACGGTTTTGCGG	AGCGAACGACAAAAAGTATGG	45.0	42.9	57.9	57.2	1320 bp	0.5	44.5
ycf53_p5	CAAAATCACGGTTTTGCGGT	GCGAACGACAAAAAGTATGGA	45.0	42.9	58.2	57.2	1318 bp	0.5	44.4