Hotspot

ycf37

Genome · 6585-7055 · 471 bp

Back to Echinothamnion

Pi 0.125

MarkerSeek score 76.40

Primer available yes

Label ycf37

Feature Metadata

label_name: ycf37
feature_type: gene
region: Genome
length_bp: 471
variable_sites: 59
pi: 0.125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf37_p1	ACCGCTTTTTGTTTTCATACGA	CGGCTTCATGATAGATCCGG	36.4	55.0	57.4	58.0	954 bp	0.5	39.5
ycf37_p2	ACCGCTTTTTGTTTTCATACGA	ACTCAGTCGGTTGGGTAAGA	36.4	50.0	57.4	58.0	1255 bp	0.5	39.5
ycf37_p3	ACCGCTTTTTGTTTTCATACGA	TTTGTGACTCAGTCGGTTGG	36.4	50.0	57.4	58.1	1261 bp	0.5	39.1
ycf37_p4	ACCGCTTTTTGTTTTCATACGA	GTCGGTTGGGTAAGAAGACC	36.4	55.0	57.4	57.9	1250 bp	0.5	38.8
ycf37_p5	ACCGCTTTTTGTTTTCATACGA	TTCAAGGACAAGGCTTAGGC	36.4	50.0	57.4	57.8	1075 bp	0.5	38.0