Hotspot

trpA

Genome · 33540-34349 · 810 bp

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Pi 0.11

MarkerSeek score 79.10

Primer available yes

Label trpA

Feature Metadata

label_name: trpA
feature_type: gene
region: Genome
length_bp: 810
variable_sites: 89
pi: 0.11
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trpA_p1	TCTTGACCGCCAGAAATAGC	TGTATATGCCCCCATCGTCT	50.0	50.0	58.0	58.0	1200 bp	0.5	59.6
trpA_p2	CTTGACCGCCAGAAATAGCT	TGTATATGCCCCCATCGTCT	50.0	50.0	58.0	58.0	1199 bp	0.5	59.6
trpA_p3	TCTTGACCGCCAGAAATAGC	TATGCCCCCATCGTCTAGAG	50.0	55.0	58.0	58.1	1196 bp	0.5	59.2
trpA_p4	CTTGACCGCCAGAAATAGCT	TATGCCCCCATCGTCTAGAG	50.0	55.0	58.0	58.1	1195 bp	0.5	59.2
trpA_p5	GAGTCTTGACCGCCAGAAAT	TGTATATGCCCCCATCGTCT	50.0	50.0	57.9	58.0	1203 bp	0.5	59.0