Hotspot

rps1

Genome · 155163-155978 · 816 bp

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Pi 0.0994

MarkerSeek score 80.10

Primer available yes

Label rps1

Feature Metadata

label_name: rps1
feature_type: gene
region: Genome
length_bp: 816
variable_sites: 81
pi: 0.0994
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps1_p1	TCCATTGCCGTAACCTCTTG	GGCGGTATATTTCTGTGGCA	50.0	50.0	57.9	58.0	1283-1299 bp	1.0	87.1
rps1_p2	TCCATTGCCGTAACCTCTTG	ATCTGGTACGCTCTTACCGA	50.0	50.0	57.9	58.0	1331-1347 bp	1.0	86.9
rps1_p3	TCCATTGCCGTAACCTCTTG	AAACATAAGCCGGGTTTGGT	50.0	45.0	57.9	58.0	1563 bp	0.5	59.1
rps1_p4	TCCATTGCCGTAACCTCTTG	AACATAAGCCGGGTTTGGTT	50.0	45.0	57.9	58.0	1562 bp	0.5	59.1
rps1_p5	TGGAGTCACAATCCATTGCC	AACATAAGCCGGGTTTGGTT	50.0	45.0	58.2	58.0	1573 bp	0.5	58.7