Hotspot

rnz

Genome · 18043-18717 · 675 bp

Back to Echinothamnion

Pi 0.08

MarkerSeek score 78.30

Primer available yes

Label rnz

Feature Metadata

label_name: rnz
feature_type: gene
region: Genome
length_bp: 675
variable_sites: 54
pi: 0.08
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rnz_p1	AATAGCTGCTGGTTCTTGGG	AGAGTTTGAATCACGGCCAT	50.0	45.0	57.9	57.5	1597-1598 bp	1.0	79.8
rnz_p2	CTGCTGGTTCTTGGGCAATA	AGAGTTTGAATCACGGCCAT	50.0	45.0	58.2	57.5	1592-1593 bp	1.0	79.6
rnz_p3	AATAGCTGCTGGTTCTTGGG	CCACCAACATCAATCACTGC	50.0	50.0	57.9	57.4	1213-1214 bp	1.0	78.9
rnz_p4	CTGCTGGTTCTTGGGCAATA	CCACCAACATCAATCACTGC	50.0	50.0	58.2	57.4	1208-1209 bp	1.0	78.7
rnz_p5	TGCTGGTTCTTGGGCAATAT	AGAGTTTGAATCACGGCCAT	45.0	45.0	57.5	57.5	1591-1592 bp	1.0	76.7