Hotspot

moeB

Genome · 149270-150331 · 1062 bp

Back to Echinothamnion

Pi 0.103

MarkerSeek score 79.10

Primer available yes

Label moeB

Feature Metadata

label_name: moeB
feature_type: gene
region: Genome
length_bp: 1062
variable_sites: 109
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
moeB_p1	ACCCAAAAATAGTTCTTCTGCT	CTACGTGCTGTGCCACTAAT	36.4	50.0	55.9	58.0	2349 bp	0.5	27.0
moeB_p2	ACCCAAAAATAGTTCTTCTGCT	AACAGAAGCTGGAGGAGTCT	36.4	50.0	55.9	58.0	2179 bp	0.5	26.9
moeB_p3	ACCCAAAAATAGTTCTTCTGCT	ACAGAAGCTGGAGGAGTCTT	36.4	50.0	55.9	58.0	2178 bp	0.5	26.9
moeB_p4	ACCCAAAAATAGTTCTTCTGCT	CTCCATGTTGACCTAACGCA	36.4	50.0	55.9	57.9	2045 bp	0.5	26.4
moeB_p5	ACCCAAAAATAGTTCTTCTGCT	TCCATGTTGACCTAACGCAG	36.4	50.0	55.9	57.9	2044 bp	0.5	26.4