Hotspot

dsbD

Genome · 23227-23964 · 738 bp

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Pi 0.0962

MarkerSeek score 78.30

Primer available yes

Label dsbD

Feature Metadata

label_name: dsbD
feature_type: gene
region: Genome
length_bp: 738
variable_sites: 71
pi: 0.0962
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dsbD_p1	AATTGCGGACGGAGAGATTC	CACCCTTAGGAACAATCTCCTG	50.0	50.0	58.1	58.1	1700 bp	0.5	42.6
dsbD_p2	GAATTGCGGACGGAGAGATT	CACCCTTAGGAACAATCTCCTG	50.0	50.0	58.1	58.1	1701 bp	0.5	42.6
dsbD_p3	AATCCTTCCTGGGGAGTTCA	CACCCTTAGGAACAATCTCCTG	50.0	50.0	57.9	58.1	1431 bp	0.5	42.6
dsbD_p4	ATCCTTCCTGGGGAGTTCAA	CACCCTTAGGAACAATCTCCTG	50.0	50.0	57.9	58.1	1430 bp	0.5	42.6
dsbD_p5	TCAAATCCTTCCTGGGGAGT	CACCCTTAGGAACAATCTCCTG	50.0	50.0	57.9	58.1	1434 bp	0.5	42.6