Hotspot

dnaB

Genome · 146903-148660 · 1758 bp

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Pi 0.111

MarkerSeek score 78.20

Primer available yes

Label dnaB

Feature Metadata

label_name: dnaB
feature_type: gene
region: Genome
length_bp: 1758
variable_sites: 195
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaB_p1	TCAGTCCACTGCTCTACCAA	GCATCACACTTTTTAGCCAACA	50.0	40.9	58.0	58.0	2511 bp	0.5	43.7
dnaB_p2	TCCACTGCTCTACCAACTGA	GCATCACACTTTTTAGCCAACA	50.0	40.9	58.0	58.0	2507 bp	0.5	43.7
dnaB_p3	TTCAGTCCACTGCTCTACCA	GCATCACACTTTTTAGCCAACA	50.0	40.9	58.0	58.0	2512 bp	0.5	43.7
dnaB_p4	GGAGATAGCGCTGTTGTTGA	GCATCACACTTTTTAGCCAACA	50.0	40.9	58.0	58.0	2721 bp	0.5	43.7
dnaB_p5	AGGAGATAGCGCTGTTGTTG	GCATCACACTTTTTAGCCAACA	50.0	40.9	58.0	58.0	2722 bp	0.5	43.7