Hotspot

ccs1

Genome · 24015-25343 · 1329 bp

Back to Echinothamnion

Pi 0.0828

MarkerSeek score 77.80

Primer available yes

Label ccs1

Feature Metadata

label_name: ccs1
feature_type: gene
region: Genome
length_bp: 1329
variable_sites: 110
pi: 0.0828
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccs1_p1	TCGTTCTGTTTACCTAAGCCA	CCGACACCTATGCATGGAAA	42.9	50.0	57.0	58.0	1977 bp	0.5	43.4
ccs1_p2	TCGTTCTGTTTACCTAAGCCA	AATATCACCAGGACCGGGAA	42.9	50.0	57.0	58.1	2122 bp	0.5	42.8
ccs1_p3	TCGTTCTGTTTACCTAAGCCA	CTACCGACACCTATGCATGG	42.9	55.0	57.0	57.8	1980 bp	0.5	42.5
ccs1_p4	TCGTTCTGTTTACCTAAGCCA	TACCGACACCTATGCATGGA	42.9	50.0	57.0	58.2	1979 bp	0.5	42.0
ccs1_p5	TCGTTCTGTTTACCTAAGCCA	CCGGGAAGACCTGAAGAATC	42.9	55.0	57.0	57.7	2109 bp	0.5	41.2