Hotspot

QQP88_pgp117

Genome · 69559-70356 · 798 bp

Back to Echinothamnion

Pi 0.158

MarkerSeek score 80.20

Primer available yes

Label QQP88_pgp117

Feature Metadata

label_name: QQP88_pgp117
feature_type: gene
region: Genome
length_bp: 798
variable_sites: 125
pi: 0.158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
QQP88_pgp117_p1	CGTTTACCGAATTTCGCCAC	ATCCATCGCTAAATGCTGCA	50.0	45.0	58.1	58.0	1537-1546 bp	1.0	87.3
QQP88_pgp117_p2	ATGCGTTTACCGAATTTCGC	ATCCATCGCTAAATGCTGCA	45.0	45.0	57.8	58.0	1540-1549 bp	1.0	86.9
QQP88_pgp117_p3	ACCGAATTTCGCCACTTACT	ATCCATCGCTAAATGCTGCA	45.0	45.0	57.5	58.0	1532-1541 bp	1.0	84.6
QQP88_pgp117_p4	CGTTTACCGAATTTCGCCAC	GATCCATCGCTAAATGCTGC	50.0	50.0	58.1	57.4	1538-1547 bp	1.0	82.6
QQP88_pgp117_p5	ATGCGTTTACCGAATTTCGC	GATCCATCGCTAAATGCTGC	45.0	50.0	57.8	57.4	1541-1550 bp	1.0	82.3