Hotspot

psaC-ycf22

Genome · 46055-46979 · 925 bp

Back to Echinothamnion

Pi 0.158

MarkerSeek score 80.10

Primer available yes

Label psaC-ycf22

Feature Metadata

label_name: psaC-ycf22
feature_type: igs
region: Genome
length_bp: 925
variable_sites: 145
pi: 0.158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ycf22_p1	AGCCGGTCAAATAGCTTCAG	ACAGAACCAACTTTCACACCT	50.0	42.9	58.0	57.6	1285 bp	0.5	48.8
psaC-ycf22_p2	AATGTGTTCGTGCTTGTCCT	ACAGAACCAACTTTCACACCT	45.0	42.9	58.0	57.6	1343 bp	0.5	48.8
psaC-ycf22_p3	ATGTGTTCGTGCTTGTCCTT	ACAGAACCAACTTTCACACCT	45.0	42.9	58.0	57.6	1342 bp	0.5	48.8
psaC-ycf22_p4	CAATGTGTTCGTGCTTGTCC	ACAGAACCAACTTTCACACCT	50.0	42.9	58.0	57.6	1344 bp	0.5	48.8
psaC-ycf22_p5	TGGTTGTAAAGCCGGTCAAA	ACAGAACCAACTTTCACACCT	45.0	42.9	57.9	57.6	1294 bp	0.5	48.6