Hotspot

ycf1

SSC · 108868-114093 · 5226 bp

Back to Echinops

Pi 0.00552

MarkerSeek score 44.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5226
variable_sites: 43
pi: 0.00552
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGACGAAAAGAACAAGGCA	TCGAGGAGGACCAAAAGGAT	45.0	50.0	58.3	58.0	829 bp	1.0	77.9
ycf1_p2	ACGACGAAAAGAACAAGGCA	GCGGAATGCTATCCCAGATT	45.0	50.0	58.3	57.8	443 bp	1.0	77.4
ycf1_p3	ACGAAAAGAACAAGGCATGC	TCGAGGAGGACCAAAAGGAT	45.0	50.0	57.6	58.0	826 bp	1.0	77.2
ycf1_p4	ACGACGAAAAGAACAAGGCA	TCGTCCAACCTGTAGATCGA	45.0	50.0	58.3	57.9	1121 bp	1.0	77.1
ycf1_p5	AACAAGGCATGCTACAAGGG	TCGAGGAGGACCAAAAGGAT	50.0	50.0	58.5	58.0	818 bp	1.0	77.0