Hotspot

petD-rpoA

LSC · 77858-78053 · 196 bp

Back to Echinops

Pi 0.0136

MarkerSeek score 62.90

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 196
variable_sites: 4
pi: 0.0136
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTGGTTGGGTATTGGTGCAA	TGGAGCATTTTCGCATAGAAGA	45.0	40.9	57.9	58.1	344-346 bp	1.0	63.9
petD-rpoA_p2	TTTGGTTGGGTATTGGTGCA	TGGAGCATTTTCGCATAGAAGA	45.0	40.9	57.9	58.1	345-347 bp	1.0	63.9
petD-rpoA_p3	TTGGTTGGGTATTGGTGCAA	TGGAGCATTTTCGCATAGAAG	45.0	42.9	57.9	56.7	344-346 bp	1.0	62.4
petD-rpoA_p4	TTTGGTTGGGTATTGGTGCA	TGGAGCATTTTCGCATAGAAG	45.0	42.9	57.9	56.7	345-347 bp	1.0	62.4
petD-rpoA_p5	CCAGTAGCGACAACCGTATT	TGGAGCATTTTCGCATAGAAGA	50.0	40.9	57.7	58.1	388-390 bp	1.0	62.1