Hotspot

ycf4-cemA

LSC · 60483-61327 · 845 bp

Back to Echinops

Pi 0.00316

MarkerSeek score 60.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 845
variable_sites: 4
pi: 0.00316
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	1025-1031 bp	1.0	79.5
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	957-963 bp	1.0	79.0
ycf4-cemA_p3	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	989-995 bp	1.0	78.9
ycf4-cemA_p4	ACAAAAAGCTGCGGAATTGG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	973-979 bp	1.0	78.5
ycf4-cemA_p5	GAGCCATTCCATTGACTCGT	AGAAATCCACCAGGGCAAAA	50.0	45.0	58.0	57.6	1024-1030 bp	1.0	76.9