Hotspot

psaA-ycf3

LSC · 41400-42147 · 748 bp

Back to Echinops

Pi 0.000917

MarkerSeek score 62.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 1
pi: 0.000917
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GAGAAATGACCCGGTCTAGC	GGAAACAGGCTATAGCGCTT	55.0	50.0	57.8	58.0	904-926 bp	1.0	79.8
psaA-ycf3_p2	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	55.0	50.0	57.8	58.3	918-940 bp	1.0	78.1
psaA-ycf3_p3	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	55.0	50.0	57.8	58.3	920-942 bp	1.0	78.1
psaA-ycf3_p4	GAGAAATGACCCGGTCTAGC	AGGCTATAGCGCTTACTCCT	55.0	50.0	57.8	57.7	898-920 bp	1.0	77.9
psaA-ycf3_p5	TTTTACTTCTGGTTCCGGCG	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.5	58.0	839-861 bp	1.0	77.9