Hotspot

psbK-psbI

LSC · 7880-8288 · 409 bp

Back to Echinops

Pi 0.00652

MarkerSeek score 53.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 409
variable_sites: 4
pi: 0.00652
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	546 bp	1.0	72.7
psbK-psbI_p2	TATGCCAGTCATACCCCTGT	TCTTCACGTCCAGGATTACG	50.0	50.0	57.8	57.1	586 bp	1.0	71.6
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	543 bp	1.0	70.7
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	544 bp	1.0	70.6
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	545 bp	1.0	70.6