Hotspot

psbA-trnK-UUU

LSC · 1542-1759 · 218 bp

Back to Echinops

Pi 0.0183

MarkerSeek score 64.20

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 218
variable_sites: 6
pi: 0.0183
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAAAGACGGTTTTCGGTGCT	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.8	57.9	420 bp	1.0	78.1
psbA-trnK-UUU_p2	TAAAGACGGTTTTCGGTGCT	CTAGTTCCGGGTTCGAATCC	45.0	55.0	57.8	57.8	335 bp	1.0	77.7
psbA-trnK-UUU_p3	GTAAAGACGGTTTTCGGTGC	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.7	57.9	421 bp	1.0	77.6
psbA-trnK-UUU_p4	GTAAAGACGGTTTTCGGTGC	CTAGTTCCGGGTTCGAATCC	50.0	55.0	57.7	57.8	336 bp	1.0	77.2
psbA-trnK-UUU_p5	TTTTCGGTGCTGGTTATCCA	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.4	57.9	411 bp	1.0	75.5