Hotspot

rpl23

LSC · 57916-58197 · 282 bp

Back to Echinochloa

Pi 0.0162

MarkerSeek score 41.70

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: LSC
length_bp: 282
variable_sites: 10
pi: 0.0162
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	GGCTTCAGGGGGTATTCATG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1250-1330 bp	1.0	85.3
rpl23_p2	GGCTTCAGGGGGTATTCATG	CTCGTTATACTCGGCTACGC	55.0	55.0	58.0	58.1	1472-1552 bp	1.0	85.0
rpl23_p3	GCCTGTGTACAAGCTCGTAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	1105-1185 bp	1.0	84.9
rpl23_p4	GGCTTCAGGGGGTATTCATG	TTTGGCTCCGTCGTTTCTAG	55.0	50.0	58.0	57.9	1307-1387 bp	1.0	84.8
rpl23_p5	GTTATACCGGTGGCTTCAGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	1336-1341 bp	0.857143	74.7