Hotspot

rpl32-trnL-UAG

SSC · 107767-108283 · 517 bp

Back to Echinochloa

Pi 0.0111

MarkerSeek score 42.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 517
variable_sites: 14
pi: 0.0111
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAAGGATTGATTTGCGGCA	GATCCGCACAAAAGAGTTGC	45.0	50.0	58.2	58.0	1393-1394 bp	1.0	79.4
rpl32-trnL-UAG_p2	AGAAGGATTGATTTGCGGCA	TCTTTTTCTAGACTGCCGCC	45.0	50.0	58.2	57.9	868-869 bp	1.0	79.4
rpl32-trnL-UAG_p3	AGAAGGATTGATTTGCGGCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	814-815 bp	1.0	79.3
rpl32-trnL-UAG_p4	AGAAGGATTGATTTGCGGCA	ATGTCCCGAAGAAACCCAAC	45.0	50.0	58.2	58.1	1127-1128 bp	1.0	79.2
rpl32-trnL-UAG_p5	AGAAGGATTGATTTGCGGCA	CCGAAGAAACCCAACGAGAA	45.0	50.0	58.2	58.1	1122-1123 bp	1.0	78.9