Hotspot

psaI-ycf4

LSC · 58695-59051 · 357 bp

Back to Echinochloa

Pi 0.00667

MarkerSeek score 48.10

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 357
variable_sites: 6
pi: 0.00667
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	TCCATATGTGTTCTGATCGCC	45.0	47.6	58.0	57.9	446-447 bp	1.0	72.0
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	CCCCAACCGCTAAGAATCC	45.0	57.9	58.0	57.9	536-537 bp	1.0	71.9
psaI-ycf4_p3	ATTTCCGGCAATTGCAATGG	CCATATGTGTTCTGATCGCCA	45.0	47.6	58.0	58.2	445-446 bp	1.0	71.6
psaI-ycf4_p4	GCAATTGCAATGGCTTCCTT	TCCATATGTGTTCTGATCGCC	45.0	47.6	57.9	57.9	439-440 bp	1.0	71.4
psaI-ycf4_p5	GCAATTGCAATGGCTTCCTT	CCCCAACCGCTAAGAATCC	45.0	57.9	57.9	57.9	529-530 bp	1.0	71.3