Hotspot

rps16-trnQ-UUG

LSC · 5454-6007 · 554 bp

Back to Echinochloa

Pi 0.0105

MarkerSeek score 47.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 554
variable_sites: 17
pi: 0.0105
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	623-627 bp	1.0	81.0
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	634-638 bp	1.0	80.4
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	627-631 bp	1.0	79.9
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	CTAAAACGAAATGGGGCGTG	45.0	50.0	58.1	57.7	682-686 bp	1.0	79.3
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	638-642 bp	1.0	79.3