Hotspot

AU144_gp009

IRa · 136112-136849 · 738 bp

Back to Echinochloa

Pi 0.00142

MarkerSeek score 49.20

Primer available yes

Label AU144_gp009

Feature Metadata

label_name: AU144_gp009
feature_type: gene
region: IRa
length_bp: 738
variable_sites: 3
pi: 0.00142
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
AU144_gp009_p1	GTAAGGATTGCACCAGAGCA	GATTGAACAACCGGGATCCA	50.0	50.0	57.9	57.9	915 bp	1.0	78.4
AU144_gp009_p2	TAAGGATTGCACCAGAGCAC	GATTGAACAACCGGGATCCA	50.0	50.0	57.9	57.9	914 bp	1.0	78.4
AU144_gp009_p3	TCAACCCGAGTGCAATCTTT	GATTGAACAACCGGGATCCA	45.0	50.0	57.7	57.9	943 bp	1.0	77.1
AU144_gp009_p4	CGGTAAGGATTGCACCAGAG	GATTGAACAACCGGGATCCA	55.0	50.0	58.3	57.9	917 bp	1.0	76.5
AU144_gp009_p5	TCTGTCGGTAAGGATTGCAC	GATTGAACAACCGGGATCCA	50.0	50.0	57.6	57.9	922 bp	1.0	76.2