Hotspot

rpl32-ndhF

SSC · 123745-124587 · 843 bp

Back to Echinacea

Pi 0.00146

MarkerSeek score 57.30

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 843
variable_sites: 4
pi: 0.00146
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CGCCCCATATCCTTTCCTTT	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.6	58.0	999-1051 bp	1.0	80.5
rpl32-ndhF_p2	CGCCCCATATCCTTTCCTTT	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	57.6	58.0	997-1049 bp	1.0	80.4
rpl32-ndhF_p3	CGCCCCATATCCTTTCCTTT	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	57.6	58.0	998-1050 bp	1.0	80.4
rpl32-ndhF_p4	TGACAAAGCTTTTAACGCCG	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.6	58.0	1017-1069 bp	1.0	80.2
rpl32-ndhF_p5	TGACAAAGCTTTTAACGCCG	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.6	58.0	1015-1067 bp	1.0	80.1