Hotspot

psbT-psbN

LSC · 73756-73828 · 73 bp

Back to Echinacea

Pi 0.0183

MarkerSeek score 51.30

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 73
variable_sites: 3
pi: 0.0183
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	GAGAACCGCCGAAAATTTCA	TCGAGGAACATGGGGACTAA	45.0	50.0	57.0	57.8	125 bp	1.0	72.1
psbT-psbN_p2	GAGAACCGCCGAAAATTTCA	TTCGAGGAACATGGGGACTA	45.0	50.0	57.0	57.8	126 bp	1.0	72.1
psbT-psbN_p3	GAACCGCCGAAAATTTCAACT	TCGAGGAACATGGGGACTAA	42.9	50.0	58.0	57.8	123 bp	1.0	72.0
psbT-psbN_p4	AGAACCGCCGAAAATTTCAAC	TCGAGGAACATGGGGACTAA	42.9	50.0	58.0	57.8	124 bp	1.0	71.9
psbT-psbN_p5	GAACCGCCGAAAATTTCAACT	TTCGAGGAACATGGGGACTA	42.9	50.0	58.0	57.8	124 bp	1.0	71.9