Hotspot

clpP-psbB

LSC · 71467-71939 · 473 bp

Back to Echinacea

Pi 0.00822

MarkerSeek score 54.50

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 10
pi: 0.00822
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	TCCTCTCCAGGACTTCGAAA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.7	58.2	538-540 bp	1.0	77.4
clpP-psbB_p2	TCCTCTCCAGGACTTCGAAA	AGCAACCAAAGCTGTATGCA	50.0	45.0	57.7	58.4	611-613 bp	1.0	76.1
clpP-psbB_p3	TCCTCTCCAGGACTTCGAAA	ACACGATACCAAGGCAAACC	50.0	50.0	57.7	58.5	541-543 bp	1.0	75.6
clpP-psbB_p4	GATGCATCTTCCTCTCCAGG	CGATACCAAGGCAAACCCAT	55.0	50.0	57.5	58.2	547-549 bp	1.0	75.5
clpP-psbB_p5	TCCTCTCCAGGACTTCGAAA	TGAACACGATACCAAGGCAA	50.0	45.0	57.7	57.4	544-546 bp	1.0	74.9