Hotspot

trnT-UGU-trnL-UAA

LSC · 46422-47008 · 587 bp

Back to Echinacea

Pi 0.00826

MarkerSeek score 60.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 587
variable_sites: 11
pi: 0.00826
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	GCGTCTACCTATTTCGCCAT	55.0	50.0	57.9	57.8	614-643 bp	1.0	83.1
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.8	57.8	646-675 bp	1.0	82.2
trnT-UGU-trnL-UAA_p3	ATGCGATGCTCTAACCTCTG	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.5	57.8	616-645 bp	1.0	80.0
trnT-UGU-trnL-UAA_p4	TGCGATGCTCTAACCTCTGA	GCGTCTACCTATTTCGCCAT	50.0	50.0	58.5	57.8	615-644 bp	1.0	80.0
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	AAGTCCGTAGCGTCTACCTA	55.0	50.0	57.9	57.3	623-652 bp	1.0	78.7