Hotspot

ycf3-trnS-GCU

LSC · 44055-44953 · 899 bp

Back to Echinacea

Pi 0.0142

MarkerSeek score 65.10

Primer available yes

Label ycf3-trnS-GCU

Feature Metadata

label_name: ycf3-trnS-GCU
feature_type: igs
region: LSC
length_bp: 899
variable_sites: 33
pi: 0.0142
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GCU_p1	TTCTCCTGAAGTTGTCGGAA	CAACCACTCAGCCATCTCTC	45.0	55.0	56.4	58.0	1012-1019 bp	1.0	70.8
ycf3-trnS-GCU_p2	TTTCTCCTGAAGTTGTCGGA	CAACCACTCAGCCATCTCTC	45.0	55.0	56.4	58.0	1013-1020 bp	1.0	70.8
ycf3-trnS-GCU_p3	TTCTCCTGAAGTTGTCGGAA	ATGCTACGCTTTCAACCACT	45.0	45.0	56.4	57.8	1024-1031 bp	1.0	69.4
ycf3-trnS-GCU_p4	TTTCTCCTGAAGTTGTCGGA	ATGCTACGCTTTCAACCACT	45.0	45.0	56.4	57.8	1025-1032 bp	1.0	69.4
ycf3-trnS-GCU_p5	TTCTCCTGAAGTTGTCGGAA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1067-1074 bp	1.0	69.0