Hotspot

psbC-trnS-UGA

LSC · 34818-35063 · 246 bp

Back to Echinacea

Pi 0.0114
MarkerSeek score 60.80
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
246
variable_sites
6
pi
0.0114
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CCTGTTCTTTCCATGACCCC GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.2 58.0 353-358 bp 1.0 81.9
psbC-trnS-UGA_p2 TCTTCGTAGGGCATTTGTGG GAGAGATGGCTGAGTGGTTG 50.0 55.0 57.9 58.0 436-441 bp 1.0 81.7
psbC-trnS-UGA_p3 CCTGTTCTTTCCATGACCCC GTCTTTCGGAGAGATGGCTG 55.0 55.0 58.2 58.1 361-366 bp 1.0 81.5
psbC-trnS-UGA_p4 TCTTCGTAGGGCATTTGTGG GTCTTTCGGAGAGATGGCTG 50.0 55.0 57.9 58.1 444-449 bp 1.0 81.3
psbC-trnS-UGA_p5 TCTTCGTAGGGCATTTGTGG TCTTTCGGAGAGATGGCTGA 50.0 50.0 57.9 58.1 443-448 bp 1.0 80.7