Hotspot

trnT-GGU-psbD

LSC · 31186-32386 · 1201 bp

Back to Echinacea

Pi 0.00244
MarkerSeek score 43.40
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1201
variable_sites
10
pi
0.00244
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTAATCCCATGGTAACGCGT GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1335-1386 bp 1.0 80.9
trnT-GGU-psbD_p2 GTAATCCCATGGTAACGCGT AAACTAGGCGCAAAGAACCA 50.0 45.0 58.1 58.0 1772-1823 bp 1.0 80.4
trnT-GGU-psbD_p3 GTAATCCCATGGTAACGCGT AACTAGGCGCAAAGAACCAA 50.0 45.0 58.1 58.0 1771-1822 bp 1.0 80.4
trnT-GGU-psbD_p4 GTAATCCCATGGTAACGCGT ACCAAAACTAGGCGCAAAGA 50.0 45.0 58.1 58.0 1776-1827 bp 1.0 80.4
trnT-GGU-psbD_p5 GTAATCCCATGGTAACGCGT TGGACCAGAGAATGCGATTG 50.0 50.0 58.1 58.0 1701-1752 bp 1.0 80.4