Hotspot

atpI-atpH

LSC · 25156-26293 · 1138 bp

Back to Echinacea

Pi 0.00132

MarkerSeek score 52.30

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1138
variable_sites: 4
pi: 0.00132
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	CCCGTCATGTTCCTTGGATT	ATAACGGAAGCGGCAGAAAT	50.0	45.0	57.9	58.0	1269-1283 bp	1.0	79.4
atpI-atpH_p2	CCCGTCATGTTCCTTGGATT	TACCTTGACCAACTCCAGGT	50.0	50.0	57.9	57.9	1325-1339 bp	1.0	78.8
atpI-atpH_p3	CCCGTCATGTTCCTTGGATT	AGCCAATCCAGCAGCAATAA	50.0	45.0	57.9	57.9	1285-1299 bp	1.0	78.5
atpI-atpH_p4	CCCGTCATGTTCCTTGGATT	AATAACGGAAGCGGCAGAAA	50.0	45.0	57.9	58.2	1270-1284 bp	1.0	78.3
atpI-atpH_p5	CCCGTCATGTTCCTTGGATT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.9	58.2	1329-1343 bp	1.0	78.2