Hotspot

ndhF

SSC · 109176-111404 · 2229 bp

Back to Echinacanthus

Pi 0.0476

MarkerSeek score 73.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 157
pi: 0.0476
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATTATGGACCCGCTACCAGA	AAAGAGGTAAGGTCCGGTCA	50.0	50.0	57.9	58.0	2685 bp	0.333333	52.4
ndhF_p2	ATTATGGACCCGCTACCAGA	AAGAGGTAAGGTCCGGTCAA	50.0	50.0	57.9	58.0	2684 bp	0.333333	52.4
ndhF_p3	GGCCCTCCTATCTCTTCCTT	AAAGAGGTAAGGTCCGGTCA	55.0	50.0	57.9	58.0	2907 bp	0.333333	52.3
ndhF_p4	GGCCCTCCTATCTCTTCCTT	AAGAGGTAAGGTCCGGTCAA	55.0	50.0	57.9	58.0	2906 bp	0.333333	52.3
ndhF_p5	TATGGACCCGCTACCAGAAA	AAGAGGTAAGGTCCGGTCAA	50.0	50.0	58.1	58.0	2682 bp	0.333333	52.1