Hotspot

ndhA

SSC · 118113-120257 · 2145 bp

Back to Echinacanthus

Pi 0.036

MarkerSeek score 70.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2145
variable_sites: 106
pi: 0.036
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CGGATTGTTCGTTGACCAGA	AGTTGAAGCTCCAAAAGGGG	50.0	50.0	58.2	58.0	2364-2511 bp	1.0	86.2
ndhA_p2	CTCGGATTGTTCGTTGACCA	AGTTGAAGCTCCAAAAGGGG	50.0	50.0	58.2	58.0	2366-2513 bp	1.0	86.2
ndhA_p3	TCGGATTGTTCGTTGACCAG	AGTTGAAGCTCCAAAAGGGG	50.0	50.0	58.2	58.0	2365-2512 bp	1.0	86.2
ndhA_p4	CGGATTGTTCGTTGACCAGA	GTTGAAGCTCCAAAAGGGGA	50.0	50.0	58.2	58.0	2363-2510 bp	1.0	86.1
ndhA_p5	TCGGATTGTTCGTTGACCAG	GTTGAAGCTCCAAAAGGGGA	50.0	50.0	58.2	58.0	2364-2511 bp	1.0	86.1