Hotspot

rpl32-trnL-UAG

SSC · 112129-112621 · 493 bp

Back to Echinacanthus

Pi 0.0914

MarkerSeek score 81.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 493
variable_sites: 63
pi: 0.0914
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGACCGGACCTTACCTCTTT	AAAAAGGCCCGAAGTAGCAA	50.0	45.0	58.0	58.0	1711 bp	0.333333	53.1
rpl32-trnL-UAG_p2	TGACCGGACCTTACCTCTTT	AAAAGGCCCGAAGTAGCAAA	50.0	45.0	58.0	58.0	1710 bp	0.333333	53.1
rpl32-trnL-UAG_p3	TTGACCGGACCTTACCTCTT	AAAAAGGCCCGAAGTAGCAA	50.0	45.0	58.0	58.0	1712 bp	0.333333	53.1
rpl32-trnL-UAG_p4	TTGACCGGACCTTACCTCTT	AAAAGGCCCGAAGTAGCAAA	50.0	45.0	58.0	58.0	1711 bp	0.333333	53.1
rpl32-trnL-UAG_p5	TGACCGGACCTTACCTCTTT	AGCAAAGCCCTGAGTGAAAA	50.0	45.0	58.0	57.9	1696 bp	0.333333	52.6