Hotspot

petA-psbJ

LSC · 63591-64603 · 1013 bp

Back to Echinacanthus

Pi 0.038

MarkerSeek score 82.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1013
variable_sites: 54
pi: 0.038
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGAAAAGGTTCAATTGGCCG	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.3	57.5	1031-1071 bp	1.0	81.8
petA-psbJ_p2	CGAAAAGGTTCAATTGGCCG	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.3	57.4	1030-1070 bp	1.0	81.3
petA-psbJ_p3	ACGTGTCCAAGGTCTTTTGT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.6	57.5	1109-1149 bp	1.0	80.4
petA-psbJ_p4	ACGTGTCCAAGGTCTTTTGT	CCGGATTAGGTTCATCCCTG	45.0	55.0	57.6	57.4	1108-1148 bp	1.0	79.9
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	TCCGGATTAGGTTCATCCCT	50.0	50.0	57.2	57.5	1115-1155 bp	1.0	77.6